Functional diversity (FD), the diversity of organisms attributes that relates to their interactions with the abiotic and biotic environments, is a core biodiversity component. FD has been increasingly assessed for the last two decades in ecology to unravel the response of species assemblages to natural or anthropogenic pressures and their effects on ecosystem functioning. FD is multifaceted and to measure its facets, several frameworks and indices have been proposed. The mFD package is an R package which provides a set of “user-friendly” functions covering all the steps of FD-based analyzes to compute 16 functional diversity indices with, in addition, customizable graphical representation of key outputs based on ggplot2 framework. mFD comes with a dedicated website that provides a set of tutorials to help new-comers in the field to compute and analyze FD .